Plasmid
Part:BBa_K530035
Designed by: Daniel Bibl Group: iGEM11_Johns_Hopkins (2011-09-24)
pRSBB416 URA3 CEN/ARS Yeast Shuttle Vector Yeast shuttle vector for propagation in E. coli and transformation into S. cerevisiae. Contains an Ampicillin resistance marker for E. coli and URA3 selectable marker for S. cerevisiae. Fully compatible for use with Standard Assembly. Contains EcoRI, XbaI, SpeI, and PstI in the multiple cloning site, in that order. This vector is a CEN/ARS episomal yeast vector.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1676
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1085
Illegal BsaI.rc site found at 3356
Illegal SapI site found at 2273
Illegal SapI site found at 4377
Illegal SapI.rc site found at 932
Plasmid Map
[edit]
Categories
Parameters
None |